Analysis of mRNA from Microdissected Frozen Tissue Sections without RNA Isolation
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چکیده
منابع مشابه
One-step RT-PCR without initial RNA isolation sStep for laser-microdissected tissue sample.
One-step RT-PCR procedure without initial RNA extraction step is tested for laser microdissected tissue sample. Unfixed cryosections of liver and kidney tissue of male SD rats were cut using laser microdissection system and directly used as templates for RT-PCR study. To check the sensitivity, 5, 25, 125, and 625 hepatocytes were cut and put in PCR-tube. After DNase treatment and cDNA synthesis...
متن کاملNOTE Pathology One-Step RT-PCR without Initial RNA Isolation Step for Laser-Microdissected Tissue Sample
One-step RT-PCR procedure without initial RNA extraction step is tested for laser microdissected tissue sample. Unfixed cryosections of liver and kidney tissue of male SD rats were cut using laser microdissection system and directly used as templates for RTPCR study. To check the sensitivity, 5, 25, 125, and 625 hepatocytes were cut and put in PCR-tube. After DNase treatment and cDNA synthesis ...
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Advances in our understanding of the expression of particular genes during the course of various human diseases have led to an increasing need to obtain high-quality and intact RNA from tissue specimens to facilitate functional genomic investigations. The emergence of laser microdissection microscopy technology has allowed researchers to undertake such studies on specific cell populations consi...
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Background and Objective: Intraoperative consultation by frozen section is a high – risk procedure with important consequences. Therefore, it is critical to determine efficiency of frozen section performance periodically. This study was performed to determine the accuracy of frozen section in Urmia University of Medical Sciences. Materials & Methods:Inthis cross sectional study, we compared th...
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Thirty years of literature have shown that changes in mRNA abundance provide insight into cellular functioning. Indeed, mRNA abundance measurements have been used to determine how effective particular drugs are in eliciting cellular responses, in monitoring behavioral responses, and in several other areas of neurobiology. Although investigation of individual mRNAs was informative, there are man...
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ژورنال
عنوان ژورنال: The American Journal of Pathology
سال: 1998
ISSN: 0002-9440
DOI: 10.1016/s0002-9440(10)65544-7